The temporal structure of the inner retina at a single glance


The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of bipolar cells stratifying at distinct depths relay the excitatory feedforward drive from photoreceptors to amacrine and ganglion cells. Current experimental techniques for studying processing in the IPL do not allow imaging the entire IPL simultaneously in the intact tissue. Here, we extend a two-photon microscope with an electrically tunable lens allowing us to obtain optical vertical slices of the IPL, which provide a complete picture of the response diversity of bipolar cells at a “single glance”. The nature of these axial recordings additionally allowed us to isolate and investigate batch effects, ie inter-experimental variations resulting in systematic differences in …

Matthias Bethge
Matthias Bethge
Professor for Computational Neuroscience and Machine Learning & Director of the Tübingen AI Center

Matthias Bethge is Professor for Computational Neuroscience and Machine Learning at the University of Tübingen and director of the Tübingen AI Center, a joint center between Tübingen University and MPI for Intelligent Systems that is part of the German AI strategy.