Two-photon laser scanning microscopy with fluorescent calcium indicators is used widely to measure the activity of large populations of neurons. Extracting biologically relevant signals of interest without manual intervention remains a challenge. Two key problems are identifying image regions corresponding to individual neurons, and then detecting the timing of individual spikes from their derived fluorescence traces. The neuroscience community still lacks automated and agreed-upon solutions to these problems. Motivated by algorithm benchmarking efforts in computer vision and machine learning, we built two web-based benchmarking systems, Neurofinder (http://neurofinder. codeneuro. org) and Spikefinder (http://spikefinder. codeneuro. org), to compare algorithm performance on standardized datasets. Both were built with modular and modern open-source tools, allowing easy reuse for other data analysis problems. Neurofinder considers the problem of identifying neuron somata in fluorescence movies. We assembled a collection of training datasets from multiple labs in a standardized format, each with labeled regions defined manually, in some cases guided by activity-independent anatomical markers. Algorithm results are submitted through a web application and evaluated on independent test data, for which labels have not been made public. Evaluation metrics separately assess accuracy of neuronal locations and shapes. Submitted results are stored in a database and metrics are presented in a leaderboard. Spikefinder considers the problem of detecting spike times from fluorescence traces, building on a recent quantitative …